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bl21 competent cells  (New England Biolabs)


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    New England Biolabs bl21 competent cells
    Bl21 Competent Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bl21+competent+e%2E+coli/BL21+Comp+E%2Ecoli/pmc13155313-202-6-9
    Average 96 stars, based on 880 article reviews
    bl21 competent cells - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection.
    Article Snippet: Fisher Scientific Cat# GIB-12309-019 Antibiotic-antimycotic Gibco Thermo Fisher Scientific Cat# GIB-15240-062 Dulbecco’s phosphate-buffered saline (D-PBS) (13), liquid Welgene Cat# LB 001-02 BL21 competent E. coli cell stock New England Biolabs Cat# C2530H Sealing film Axygen Cat# PCR-SP m-clear F-bottom black plates Greiner Greiner CELLSTAR microplate 781090 Protein purification column Bio-Rad Poly-prep chromatography column 7311550 Protein conc



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    Image Search Results


    Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

    Journal: Frontiers in Veterinary Science

    Article Title: Construction and immunogenicity evaluation of a bivalent nanoparticle based on mi3 displaying porcine circovirus type 2 and type 3 capsid proteins

    doi: 10.3389/fvets.2026.1862938

    Figure Lengend Snippet: Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

    Article Snippet: The pET28a(+) plasmid for recombinant protein expression and BL21(DE3) E. coli competent cells were purchased from Sangon Biotech (Shanghai) Co., Ltd.

    Techniques: Protein Purification, Clone Assay, Expressing, Purification, Recombinant, Sonication, Western Blot, Biomarker Discovery

    Expression and purification of Nb16-β-gal. BL21 bacteria carrying pRCH-Nb16-βGal were used to produce the target protein. Proteins in lysates from uninduced (lane U) and induced (lane I) bacteria, fractions containing the target protein after Ni 2+ column purification (lane 1), and the purified target protein after the second-step SEC purification (lane 2) were analyzed by SDS-PAGE on a 4–12% SDS gel. The molecular masses (in kDa) of the proteins in the protein standards (lane M) are indicated on the right side of the gel image. The horizontal arrow indicates the Nb16-β-gal band.

    Journal: Food Chemistry: Molecular Sciences

    Article Title: Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen

    doi: 10.1016/j.fochms.2026.100357

    Figure Lengend Snippet: Expression and purification of Nb16-β-gal. BL21 bacteria carrying pRCH-Nb16-βGal were used to produce the target protein. Proteins in lysates from uninduced (lane U) and induced (lane I) bacteria, fractions containing the target protein after Ni 2+ column purification (lane 1), and the purified target protein after the second-step SEC purification (lane 2) were analyzed by SDS-PAGE on a 4–12% SDS gel. The molecular masses (in kDa) of the proteins in the protein standards (lane M) are indicated on the right side of the gel image. The horizontal arrow indicates the Nb16-β-gal band.

    Article Snippet: Competent E. coli BL21 (BL21 StarTM (DE3), Fisher Scientific) was transformed with each of the plasmids described above.

    Techniques: Expressing, Purification, Bacteria, SDS Page, SDS-Gel